New strains of plant growth-promoting rhizobacteria in combinations with humic acid to enhance squash growth under saline stress

The study aims at assessing the potentials of some new salt-tolerant isolates as plant growth-promoting rhizobacteria (PGPR) under saline condition. Three 165 that grew on presence 2-20% NaCl were high and had many features PGPR. They identified by using 16S rRNA gene sequencing. nearest species to our Paenibacillus polymyxa (GQ375783.1), Ochrobactrum intermedium (MG309678.1) Enterobacter cloacae (MG309676.1) with nucleotide similarity 99, 97 99%, respectively. In 2017, a greenhouse trial was carried out assess efficiency these novice combined humic acid doses inorganic fertilizers squash (Cucurbita pepo L.) growth productivity. Data showed fertilizing soil full dose chemical only lead decrease values dehydrogenase, alkaline phosphatase nitrogenase activity all determination periods. While, inoculation PGPR strains NPK 50% spraying gave higher records enzymes activity. Moreover, data highest peroxidase polyphenol oxidase observed in sprayed inoculated half mineral-NPK. Generally, inoculating has positive effect nutrients uptake, characteristics yield components well fruits quality. So, it could be recommended biofertilizers promote growth, increase crop production salinity condition, costs reduce pollution